Volume 24, Number 2
Fall 2007

Vernier Software & Technology

The Caliper is a publication for users of Vernier products

Improved CO2 Gas Sensor Allows for Studies of Human Respiration

CO2 Gas Sensor in a BioChamber 250
CO2 Gas Sensor in a BioChamber 250

We all know that when you inhale, some of the oxygen gas in that breath is converted into carbon dioxide gas and then exhaled. But how much CO2 gas is produced? Would more CO2 gas be produced if you held your breath before exhaling? We used our new CO2 Gas Sensor on its high-range setting and a BioChamber 250 to answer these questions.

First, a normal breath was exhaled through the neck of the BioChamber 250, filling the chamber. A #6 rubber stopper was placed in the top vent and a CO2 Gas Sensor was placed in the neck, as shown.

Logger Pro data of human respiration
Logger Pro data of human respiration

Data collection was initiated with the results shown. This normal breath produced a CO2 gas concentration of around 48,000 ppm. For the second run, the subject held her breath for 30 seconds prior to exhaling into the chamber. A CO2 gas concentration of 65,000 ppm was measured. The final run, with the subject holding her breath for 60 seconds, produced a CO2 gas concentration of 76,000 ppm. Clearly, more CO2 gas was being produced the longer she held her breath. Was the oxygen gas being consumed in the same way? She'll have to get out her Vernier Oxygen Gas Sensor and start experimenting!

CO2 and O2 Graph
CO2 and O2

BioChambers Enhance Experimentation

Many of you have used our CO2 Gas Sensors to study the respiration rate of germinating peas. Now, with the BioChamber 250, it is easy to connect an O2 Gas Sensor simultaneously as shown below. Simply soak some peas for a few hours and place them in the chamber. Connect the two sensors and begin data collection. The graph below shows the impressive results, utilizing Logger Pro's capability of adding a second y-axis on the right side.

The BioChamber 2000, the 2 liter version shown below, opens the door to an unlimited variety of experiments with its larger volume and easy-access lid.

CO2, O2, and BioChamber 250
Two sensors in BioChamber 250
CO2, O2, and BioChamber 2000
Two sensors in BioChamber 2000
Table of Contents