Diffusion
Recommended for grades 10–16.

Introduction
Diffusion is a process that allows ions or molecules to move from where they are more concentrated to where they are less concentrated. This process accounts for the movement of many small molecules across a cell membrane. Diffusion is one of the processes by which cells acquire food and exchange waste products. Oxygen, for instance, might diffuse in pond water for use by fish and other aquatic animals. When animals use oxygen, more oxygen will diffuse to replace it from the neighboring environment. Waste products released by aquatic animals are diluted by diffusion and dispersed throughout a pond.
One way to measure the rate of diffusion of ions is to monitor their concentration in solution over a period of time. Since ions are electrically charged, aqueous solutions containing ions will conduct electricity. A Conductivity Probe is capable of monitoring ions in solution. This probe however, will not measure the amount of electrically neutral molecules dissolved in water. Salts, such as sodium chloride, produce ions when they dissolve in water. An equation for the dissociation of sodium chloride in water can be written as follows:
If you place a salt solution in a container such as dialysis tubing, the salt can travel through the very small holes in the tubing. When dialysis tubing containing a solution of salt ions is placed into a beaker of water, the ions can diffuse out of the tubing and into the surrounding water. In this way, you will be able to measure the diffusion of salts in a solution of water and determine how concentration gradients and the presence of other particles affect the diffusion of the salt across a membrane.
Objectives
After completing the Preliminary Activity, you will first use reference sources to find out more about diffusion and diffusion through a membrane before you choose and investigate a researchable question dealing with diffusion rate. Some topics to consider in your reference search are:
- diffusion
- facilitated diffusion
- concentration gradient
- salts
- ion channels
- ions
- dialysis
- semipermeable membrane
- active transport
Sensors and Equipment
This investigation requires each of the following Vernier sensors and equipment (unless otherwise noted):
Additional Requirements
You may also need an interface and software for data collection. What do I need for data collection?
Download Experiment Preview
The student-version preview includes:
- Step-by-step instructions for the Preliminary Activity
- List of materials and equipment
Note: The experiment preview of the Preliminary Activity does not include essential teacher information, safety tips, or sample data. Instructions for Logger Pro and other software (such as LabQuest App or TI handheld software, where available) are on the CD that accompanies the book. We strongly recommend that you purchase the book before performing experiments.
Standards Correlations
No standards correlations for this experiment.

