Tips for using the CO2-O2 Tee

The CO2-O2 Tee is designed to be used with a CO2 Gas Sensor, an O2 Gas Sensor and a 250 mL Nalgene bottle. The tapered end of the Tee fits into the neck of the Nalgene bottle. The CO2 Gas Sensor should be put in the Tee directly across from the bottle. The O2 Gas Sensor fits snugly in the top of the Tee. The entire assembly should be positioned so that the bottle and CO2 Gas Sensor are horizontal and the O2 Gas Sensor is vertical.

You may need to put a layer of tape around the tapered end of the Tee to produce a tight fit. Tolerances for the Nalgene bottles are set to the outer diameter of the opening so inside diameters may vary.

When using the Tee for experiments such as cell respiration and photosynthesis you may need to wait a few extra minutes for the gases to permeate through the Tee.