Vernier Software and Technology
Vernier Software & Technology

Enzyme Analysis using Tyrosinase

Figure from experiment 15 from Advanced Biology with Vernier


Enzymes are molecules that regulate the chemical reactions that occur in all living organisms. Almost all enzymes are globular proteins that act as catalysts, substances that speed up chemical reactions. Enzymes catalyze reactions by reducing the activation energy for a specific reaction to occur and yet are neither destroyed nor altered during this process. At the molecular level, enzymes catalyze these reactions by briefly binding to the substrate or reactants to form an enzyme-substrate complex. The reaction takes place while the substrate is bound to the enzyme, converting the substrate to the new product. The new product is then released from the enzyme substrate complex and the enzyme is then free to bind with more substrate.

{\text{Enzyme  +  Substrate }} \to {\text{ Enzyme - Substrate Complex }} \to {\text{ Enzyme  +  Product}}

Based on this model, the rate at which the product can be produced depends on the amount of enzyme and substrate that are present during the reaction. If there is excess substrate and a small amount of enzyme in solution, the reaction rate, or velocity of the reaction, will increase with the amount of enzyme in the solution. In this case, all of the enzyme molecules are busy catalyzing reactions even though there is still plenty of substrate that can be turned into product. The velocity of the reaction can only increase if the concentration of enzyme is increased. Put another way, the velocity of the reaction should increase in direct proportion to the concentration of enzyme in solution.


In this experiment, you will

  • Observe and compare the reaction rate of two substrates, tyrosine and DOPA.
  • Determine the effect of increasing enzyme concentration on the reaction rate of an enzyme at a given substrate concentration.
  • Determine the effect of increasing substrate concentration on the reaction rate of an enzyme at a given enzyme concentration.
  • Estimate the parameters, Vmax, ½Vmax, and Km for your enzyme extract.

Sensors and Equipment

This experiment features the following Vernier sensors and equipment.

Additional Requirements

You may also need an interface and software for data collection. What do I need for data collection?

Standards Correlations

See all standards correlations for Advanced Biology with Vernier »

Advanced Biology with Vernier

See other experiments from the lab book.

1ADiffusion through Membranes
2AEnzyme Action: Testing Catalase Activity
2BEnzyme Action: Testing Catalase Activity
3Mitosis & Meiosis
4APlant Pigment Chromatography
5ACell Respiration (CO2 and O2)
5BCell Respiration (CO2)
5CCell Respiration (O2)
5DCell Respiration (Pressure)
6ApGLO™ Bacterial Transformation
6BAnalysis of Precut Lambda DNA
6BForensic DNA Fingerprinting
7Genetics of Drosophila
8Population Genetics and Evolution
10ABlood Pressure as a Vital Sign
10BHeart Rate and Physical Fitness
11Animal Behavior
12ADissolved Oxygen in Water
12BPrimary Productivity
13The Visible Spectra of Plant Pigments
14Determination of Chlorophyll in Olive Oil
15Enzyme Analysis using Tyrosinase
16Introduction to Neurotransmitters using AChE
17Macromolecules: Experiments with Protein

Experiment 15 from Advanced Biology with Vernier Lab Book

<i>Advanced Biology with Vernier</i> book cover

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