Vernier Software and Technology
Vernier Software & Technology

Enzyme Action: Testing Catalase Activity

Introduction

Many organisms can decompose hydrogen peroxide (H2O2) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities of living organisms. They act as catalysts, as substances that speed up chemical reactions without being destroyed or altered during the process. Enzymes are extremely efficient and may be used over and over again. One enzyme may catalyze thousands of reactions every second. Both the temperature and the pH at which enzymes function are extremely important. Most organisms have a preferred temperature range in which they survive, and their enzymes most likely function best within that temperature range. If the environment of the enzyme is too acidic or too basic, the enzyme may irreversibly denature, or unravel, until it no longer has the shape necessary for proper functioning.

H2O2 is toxic to most living organisms. Many organisms are capable of enzymatically destroying the H2O2 before it can do much damage. H2O2 can be converted to oxygen and water, as follows:

{\text{2 }}{{\text{H}}_{\text{2}}}{{\text{O}}_{\text{2}}} \to {\text{2 }}{{\text{H}}_{\text{2}}}{\text{O }} + {\text{ }}{{\text{O}}_{\text{2}}}

Although this reaction occurs spontaneously, the enzyme catalase increases the rate considerably. Catalase is found in most living organisms. A great deal can be learned about enzymes by studying the rates of enzyme-catalyzed reactions.

Objectives

In this experiment, you will

  • Use a Gas Pressure Sensor to measure the production of oxygen gas as hydrogen peroxide is destroyed by the enzyme catalase or peroxidase at various enzyme concentrations.
  • Measure and compare the initial rates of reaction for this enzyme when different concentrations of enzyme react with H2O2.
  • Measure the production of oxygen gas as hydrogen peroxide is destroyed by the enzyme catalase or peroxidase at various temperatures.
  • Measure and compare the initial rates of reaction for the enzyme at each temperature.
  • Measure the production of oxygen gas as hydrogen peroxide is destroyed by the enzyme catalase or peroxidase at various pH values.
  • Measure and compare the initial rates of reaction for the enzyme at each pH value.

Sensors and Equipment

This experiment features the following Vernier sensors and equipment.

Option 1

Option 2

Additional Requirements

You may also need an interface and software for data collection. What do I need for data collection?

Agricultural Science with Vernier

See other experiments from the lab book.

1Introduction to Data Collection
2Acids and Bases
3Diffusion through Membranes
4Conducting Solutions
5Osmosis
6Respiration of Sugars by Yeast
7Reflection and Absorption of Light
8Soil pH
9Soil Salinity
10Soil Temperature
11Soil Moisture
12APhotosynthesis and Respiration (CO2)
12BPhotosynthesis and Respiration (O2)
12CPhotosynthesis and Respiration (CO2 and O2)
13Transpiration
14ACell Respiration (CO2)
14BCell Respiration (O2)
14CCell Respiration (CO2 and O2)
15The Greenhouse Effect
16Energy in Food
17AEnzyme Action: Testing Catalase Activity
17BEnzyme Action: Testing Catalase Activity
18ALactase Action
18BLactase Action
19Oxygen Gas and Human Respiration
20Biochemical Oxygen Demand
21Animal Temperature
22Lemon "Juice"
23Ohm's Law
24Energy Content of Fuels
25Photovoltaic Cells
26Wind Power
27Watershed Testing
28Interdependence of Plants and Animals
29Biodiversity and Ecosystems

Experiment 17B from Agricultural Science with Vernier Lab Book

<em>Agricultural Science with Vernier</em> book cover

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