Vernier Software and Technology
Vernier Software & Technology

Analysis of Precut Lambda DNA

Figure from experiment 6B from Advanced Biology with Vernier

Introduction

Restriction enzymes are a special class of proteins that cut DNA at specific sites and have become an indispensable tool in molecular biology. Restriction enzymes, also known as endonucleases, recognize specific sequences of DNA base pairs and cut or chemically separate the DNA at that site. The specific sequence of DNA recognized by a restriction enzyme is called a restriction site.

These unique enzymes occur naturally in some bacteria and act to protect them from invading viruses. Viruses called bacteriophages, phages for short, attack bacteria by inserting their genetic material into the bacterial cell. The phage commandeers the bacterial cell, replicating rapidly until the bacterial cell lyses and releases more phages to carry out the same infection process in neighboring cells. However, if the bacterial strain has restriction enzymes that recognize restriction sites on the invading phage nucleic acid, then enzymes can destroy the invading genetic material by digesting and inactivating the phage genes. Bacterial cells protect their own DNA from being self-digested by modifying certain nitrogen bases along their genome, this prevents their restriction enzymes from recognizing and digesting their own sequences.

Objectives

In this experiment, you will

  • Perform agarose gel electrophoresis using three different predigested samples of lambda DNA and uncut lambda DNA.
  • Stain the gel.
  • Document and examine gel results with an imaging system.
  • Construct a standard curve and determine the size of the DNA fragments from the gel using Logger Pro.

Sensors and Equipment

This experiment features the following Vernier sensors and equipment.

Additional Requirements

You may also need an interface and software for data collection. What do I need for data collection?

Download Complete Experiment

The downloadable file contains the student instructions in PDF and Word format as well as the teacher information.

Download Experiment

Standards Correlations

See all standards correlations for Advanced Biology with Vernier »

Advanced Biology with Vernier

See other experiments from the lab book.

1ADiffusion through Membranes
1BOsmosis
2AEnzyme Action: Testing Catalase Activity
2BEnzyme Action: Testing Catalase Activity
3Mitosis & Meiosis
4APlant Pigment Chromatography
4BPhotosynthesis
5ACell Respiration (CO2 and O2)
5BCell Respiration (CO2)
5CCell Respiration (O2)
5DCell Respiration (Pressure)
6ApGLO™ Bacterial Transformation
6BAnalysis of Precut Lambda DNA
6BForensic DNA Fingerprinting
7Genetics of Drosophila
8Population Genetics and Evolution
9Transpiration
10ABlood Pressure as a Vital Sign
10BHeart Rate and Physical Fitness
11Animal Behavior
12ADissolved Oxygen in Water
12BPrimary Productivity
13The Visible Spectra of Plant Pigments
14Determination of Chlorophyll in Olive Oil
15Enzyme Analysis using Tyrosinase
16Introduction to Neurotransmitters using AChE
17Macromolecules: Experiments with Protein

Experiment 6B from Advanced Biology with Vernier Lab Book

<i>Advanced Biology with Vernier</i> book cover

Included in the Lab Book

Vernier lab books include word-processing files of the student instructions, essential teacher information, suggested answers, sample data and graphs, and more.

Buy the Book

Go to top