Vernier Software and Technology
Vernier Software & Technology

Buffer Investigations

Figure from experiment 1 from Investigating Biology through Inquiry


Organisms are often very sensitive to the effect of acids and bases in their environment. They need to maintain a stable internal pH in order to survive—even in the event of environmental changes. Many naturally occurring biological, geological, and human-made chemicals are capable of stabilizing the environment’s pH in a process called buffering. This may allow organisms to better survive in diverse environments found throughout the earth.

A buffer is a mixture of a weak acid and its conjugate base, or a weak base and its conjugate acid. A buffer’s function is to absorb acids (H+ or H3O+ ions) or bases (OH ions) so that pH changes very, very little. The bicarbonate-carbon dioxide buffer system, for example, keeps the pH of blood in humans between 7.3 and 7.5.

Baking soda, like many substances, has some buffering ability. In the Preliminary Activity, you will determine the buffering ability of a baking soda solution. You will first use a pH Sensor to monitor pH as you add hydrochloric acid (HCl) to one portion of the sample. Subsequently, you will monitor pH as you add basic sodium hydroxide (NaOH) solution to another portion of the sample. When data collection is complete, you will determine the total pH change of the baking soda sample.

Sensors and Equipment

This investigation features the following Vernier sensors and equipment.

Additional Requirements

You may also need an interface and software for data collection. What do I need for data collection?

Investigating Biology through Inquiry

See other experiments from the lab book.

1Buffer Investigations
3Investigating Osmosis
4Chemistry of Membranes
5Investigating Protein: The Bradford Assay
6ATesting Catalase Activity (O2 Gas Sensor)
6BTesting Catalase Activity (Gas Pressure)
7Introduction to Biofuels: Enzyme Action
8Analysis of Enzymes using Tyrosinase
9Cell Respiration
10Sugar Metabolism with Yeast
11Fermentation with Yeast
12Photosynthesis by Chloroplasts
13Transpiration of Plants
14Plant Pigments
15Heart Rate
16Investigating Dissolved Oxygen
17Investigating Primary Productivity
18Modeling Population Dynamics
19Long Term Water Monitoring
20Evolution of Cellobiase
21Introduction to Molecular Evolution
22Evolution of Yeast

Investigation 1 from Investigating Biology through Inquiry Lab Book

<i>Investigating Biology through Inquiry</i> book cover

Included in the Lab Book

Vernier lab books include word-processing files of the student instructions, essential teacher information, suggested answers, sample data and graphs, and more.

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