Vernier Software and Technology
Vernier Software & Technology

Photosynthesis by Chloroplasts

Figure from experiment 12 from Investigating Biology through Inquiry

Introduction

The process of photosynthesis involves the use of light energy to convert carbon dioxide and water into sugar, oxygen, and other organic compounds. This process is often summarized by the following reaction:

\text{6 } \text{H}_{2}\text{O} + \text{6 } \text{CO}_{2} + \text{light energy} \rightarrow \text{C}_{6}\text{H}_{12}\text{O}_{6} + \text{6 } \text{O}_{2}

This process is an extremely complex one, occurring in two stages. The first stage, called the light reactions of photosynthesis, requires light energy. The products of the light reactions are then used to produce glucose from carbon dioxide and water. Because the reactions in the second stage do not require the direct use of light energy, they are called the dark reactions of photosynthesis.

In the light reactions, electrons derived from water are “excited” (raised to higher energy levels) in several steps, called photosystems I and II. In both steps, chlorophyll absorbs light energy that is used to excite the electrons. Normally, these electrons are passed to a cytochrome-containing electron transport chain. In the first photosystem, these electrons are used to generate ATP. In the second photosystem, excited electrons are used to produce the reduced coenzyme nicotinamide adenine dinucleotide phosphate (NADPH). Both ATP and NADPH are then used in the dark reactions to produce glucose.

In this investigation, a blue dye (2,6-dichlorophenol-indophenol, or DPIP) will be used to replace NADPH in the light reactions. When the dye is oxidized, it is blue. When reduced, however, it turns colorless. Since DPIP replaces NADPH in the light reactions, it will turn from blue to colorless when reduced during photosynthesis.

Objectives

In the Preliminary Activity, you will use a spectrophotometer to measure color changes in DPIP due to photosynthesis by chloroplasts.

After completing the Preliminary Activity, you will first use reference sources to find out more about photosynthesis by chloroplasts before you choose and investigate a researchable question dealing with photosynthesis. Some topics to consider in your reference search are:

  • photosynthesis
  • chlorophyll
  • chloroplast
  • ATP
  • NADPH
  • electron transport chain

Sensors and Equipment

This investigation features the following Vernier sensors and equipment.

Additional Requirements

You may also need an interface and software for data collection. What do I need for data collection?

Investigation 12 from Investigating Biology through Inquiry Lab Book

<i>Investigating Biology through Inquiry</i> book cover

Included in the Lab Book

Vernier lab books include word-processing files of the student instructions, essential teacher information, suggested answers, sample data and graphs, and more.

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