Vernier Software and Technology
Vernier Software & Technology

Testing Enzyme Activity (Spectrometer)

Introduction

Enzymes are molecules that regulate the chemical reactions that occur sin all living organisms. Almost all enzymes are globular proteins that act as catalysts, substances that speed up chemical reactions. Enzymes catalyze reactions by reducing the activation energy for a specific reaction to occur and yet are neither destroyed nor altered during this process. At the molecular level, enzymes catalyze these reactions by briefly binding to the substrate or reactants to form an enzyme-substrate complex. The reaction takes place while the substrate is bound to the enzyme, converting the substrate to the new product. The new product is then released from the enzyme substrate complex and the enzyme is then free to bind with more substrate.

{\text{Enzyme}} + {{\text{Substrate}}} \to {{\text{Enzyme-Substrate Complex}}} \to {\text{Enzyme}} + {{\text{Product}}}

H2O2 is toxic to most living organisms. Many organisms are capable of enzymatically destroying the H2O2 before it can do much damage. H2O2 can be converted to oxygen and water as follows:

{\text{2}}\ {\text{H}}_{\text{2}}{\text{O}}_{\text{2}}{\text{(aq)}} \to {\text{2}}\ {\text{H}}_{\text{2}}{\text{O}} + {\text{O}}_{\text{2}}{\text{(g)}}

Although this reaction occurs spontaneously, enzymes increase the rate considerably. At least two different enzymes are known to catalyze this reaction: catalase, found in animals and protists, and peroxidase, found in plants. A great deal can be learned about enzymes by studying the rates of enzyme-catalyzed reactions.

In this Preliminary Activity, you will use a colorimetric assay to determine the rate of reaction of the enzyme peroxidase.

After completing the Preliminary Activity, you will use reference sources to find out more about enzymes, turnips, and peroxidase, and then you will choose and investigate a researchable question. Some topics to consider in your reference search include the following:

  • catalyst
  • enzyme
  • substrate
  • peroxidase
  • proteins
  • denature
  • Michaelis-Menten constant
  • Lineweaver-Burk plot

Objectives

  • Identify variables, design and perform the investigation, collect data, analyze data, draw a conclusion, and formulate a knowledge claim based on evidence from the investigation.
  • Determine the rate of the peroxidase catalyzed reaction converting H2O2 to H2O and O2.
  • Gain increased understanding of factors affecting peroxidase activity.

Sensors and Equipment

This investigation features the following Vernier sensors and equipment.

Additional Requirements

You may also need an interface and software for data collection. What do I need for data collection?

Investigating Biology through Inquiry

See other experiments from the lab book.

1Buffer Investigations
2Diffusion
3Investigating Osmosis
4Chemistry of Membranes
5Investigating Protein
6ATesting Catalase Activity (O2 Gas Sensor)
6BTesting Catalase Activity (Gas Pressure)
6CTesting Enzyme Activity (Spectrometer)
7Introduction to Biofuels: Enzyme Action
8Analysis of Enzymes using Tyrosinase
9Cellular Respiration
10ASugar Metabolism with Yeast (Carbon Dioxide Gas)
10BSugar Metabolism with Yeast (Ethanol)
11Fermentation with Yeast
12Photosynthesis by Chloroplasts
13Transpiration of Plants
14Plant Pigments
15Heart Rate
16Investigating Dissolved Oxygen
17Investigating Primary Productivity
18Modeling Population Dynamics
19Water Monitoring
20Evolution of Cellobiase in Fungi
21Introduction to Molecular Evolution
22Evolution of Yeast

Investigation 6C from Investigating Biology through Inquiry Lab Book

<i>Investigating Biology through Inquiry</i> book cover

Included in the Lab Book

Vernier lab books include word-processing files of the student instructions, essential teacher information, suggested answers, sample data and graphs, and more.

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