Vernier Software & Technology

# Cell Respiration (Pressure)

## Introduction

Cell respiration refers to the process of converting the chemical energy of organic molecules into a form immediately usable by organisms. Glucose may be oxidized completely if sufficient oxygen is available, by the following equation:

${{\text{C}}_{\text{6}}}{{\text{H}}_{{\text{12}}}}{{\text{O}}_{\text{6}}} + {\text{ 6 }}{{\text{O}}_{\text{2}}}\left( {\text{g}} \right) \to {\text{6 }}{{\text{H}}_{\text{2}}}{\text{O }} + {\text{ 6 C}}{{\text{O}}_{\text{2}}}\left( {\text{g}} \right) + {\text{ energy}}$

All organisms, including plants and animals, oxidize glucose for energy. Often, this energy is used to convert ADP and phosphate into ATP.

To measure the rate of cell respiration, the pressure change due to the consumption of oxygen by peas will be measured. It is not possible to directly measure pressure changes due to oxygen, since the pressure sensor measures the total pressure change. Carbon dioxide is produced as oxygen is consumed. The pressure due to CO2 might cancel out any change due to the consumption of oxygen. To eliminate this problem, a chemical will be added that will selectively remove CO2. Potassium hydroxide, KOH, will chemically react with CO2 by the following equation:

${\text{2 KOH }} + {\text{ C}}{{\text{O}}_{\text{2}}} \to {{\text{K}}_{\text{2}}}{\text{C}}{{\text{O}}_{\text{3}}} + {{\text{H}}_{\text{2}}}{\text{O}}$

This will allow you to monitor pressure changes exclusively due to the consumption of oxygen.

## Objectives

In this experiment, you will

• Measure gas production.
• Study the effect of temperature on cell respiration.
• Determine whether germinating peas and non-germinating peas respire.
• Compare the rates of cell respiration in germinating and non-germinating peas.

## Sensors and Equipment

This experiment features the following Vernier sensors and equipment.

### Option 2

You may also need an interface and software for data collection. What do I need for data collection?

## Biology with Vernier

See other experiments from the lab book.

 1 Energy in Food 2 Limitations on Cell Size: Surface Area to Volume 3 Acids and Bases 4 Diffusion through Membranes 5 Conducting Solutions 6A Enzyme Action: Testing Catalase Activity 6B Enzyme Action: Testing Catalase Activity 7 Photosynthesis 8 The Effect of Alcohol on Biological Membranes 9 Biological Membranes 10 Transpiration 11A Cell Respiration (O2) 11B Cell Respiration (CO2) 11C Cell Respiration (Pressure) 11D Cell Respiration (CO2 and O2) 12A Respiration of Sugars by Yeast 12B Sugar Fermentation 13 Population Dynamics 14 Interdependence of Plants and Animals 15 Biodiversity and Ecosystems 16A Effect of Temperature on Respiration 16B Effect of Temperature on Fermentation 17 Aerobic Respiration 18 Acid Rain 19 Dissolved Oxygen in Water 20 Watershed Testing 21 Physical Profile of a Lake 22 Osmosis 23A Effect of Temperature on Cold-Blooded Organisms 23B Effect of Temperature on Cold-Blooded Organisms 24A Lactase Action 24B Lactase Action 25 Primary Productivity 26 Control of Human Respiration 27 Heart Rate and Physical Fitness 28 Monitoring EKG 29 Ventilation and Heart Rate 30 Oxygen Gas and Human Respiration 31A Photosynthesis and Respiration (O2) 31B Photosynthesis and Respiration (CO2) 31C Photosynthesis and Respiration (CO2 and O2)

### Experiment 11C from Biology with Vernier Lab Book

#### Included in the Lab Book

Vernier lab books include word-processing files of the student instructions, essential teacher information, suggested answers, sample data and graphs, and more.