Vernier Software and Technology
Vernier Software & Technology

Effect of Temperature on Respiration

Figure from experiment 16A from Biology with Vernier

Introduction

Temperature changes have profound effects upon living things. Enzyme-catalyzed reactions are especially sensitive to small changes in temperature. Because of this, the metabolism of poikilotherms, organisms whose internal body temperature is determined by their surroundings, are often determined by the surrounding temperature. Bakers who use yeast in their bread making are very aware of this. Yeast is used to leaven bread (make it rise). Yeast leavens bread by fermenting sugar, producing carbon dioxide, CO2, as a waste product. Some of the carbon dioxide is trapped by the dough and forms small “air” pockets that make the bread light. If the yeast is not warmed properly, it will not be of much use as a leavening agent; the yeast cells will burn sugar much too slowly. In this experiment, you will watch yeast cells respire (burn sugar) at different temperatures and measure their rates of respiration. Each team will be assigned three temperatures by your teacher and will share their results with other class members.

You will observe the yeast under aerobic conditions and monitor the change in concentration of carbon dioxide released by the yeast. When yeast burn sugar under anaerobic conditions, ethanol (ethyl alcohol) and carbon dioxide are released in a process called fermentation. When yeast burn sugar under aerobic conditions, water and carbon dioxide are released in a process known as oxidative respiration. Thus, the metabolic activity of yeast may be measured by monitoring the concentration of carbon dioxide produced during a specific period of time.

Objectives

In this experiment, you will

  • Use a CO2 Gas Sensor to measure the concentration of carbon dioxide produced during respiration.
  • Determine the rate of respiration of yeast at different temperatures.

Sensors and Equipment

This experiment features the following Vernier sensors and equipment.

Option 1

Option 2

Additional Requirements

You may also need an interface and software for data collection. What do I need for data collection?

Standards Correlations

See all standards correlations for Biology with Vernier »

Biology with Vernier

See other experiments from the lab book.

1Energy in Food
2Limitations on Cell Size: Surface Area to Volume
3Acids and Bases
4Diffusion through Membranes
5Conducting Solutions
6AEnzyme Action: Testing Catalase Activity
6BEnzyme Action: Testing Catalase Activity
7Photosynthesis
8The Effect of Alcohol on Biological Membranes
9Biological Membranes
10Transpiration
11ACell Respiration (O2)
11BCell Respiration (CO2)
11CCell Respiration (Pressure)
11DCell Respiration (CO2 and O2)
12ARespiration of Sugars by Yeast
12BSugar Fermentation
13Population Dynamics
14Interdependence of Plants and Animals
15Biodiversity and Ecosystems
16AEffect of Temperature on Respiration
16BEffect of Temperature on Fermentation
17Aerobic Respiration
18Acid Rain
19Dissolved Oxygen in Water
20Watershed Testing
21Physical Profile of a Lake
22Osmosis
23AEffect of Temperature on Cold-Blooded Organisms
23BEffect of Temperature on Cold-Blooded Organisms
24ALactase Action
24BLactase Action
25Primary Productivity
26Control of Human Respiration
27Heart Rate and Physical Fitness
28Monitoring EKG
29Ventilation and Heart Rate
30Oxygen Gas and Human Respiration
31APhotosynthesis and Respiration (O2)
31BPhotosynthesis and Respiration (CO2)
31CPhotosynthesis and Respiration (CO2 and O2)

Experiment 16A from Biology with Vernier Lab Book

<em>Biology with Vernier</em> book cover

Included in the Lab Book

Vernier lab books include word-processing files of the student instructions, essential teacher information, suggested answers, sample data and graphs, and more.

Buy the Book

Dev Reference: VST0053

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