Vernier Software and Technology
Vernier Software & Technology

Synthesis of Fluorescein

Introduction

Fluorescein is an organic fluorescent dye used in several areas, ranging from medicine to research applications. Structurally, fluorescein is similar to phenolphthalein, a well known dye indicator used in acid-base titrations. Fluorescein is fluorescent whereas phenolphthalein is not.

Fluorescence occurs due to the emission of light from the relaxation of the molecule from the excited state to the ground state. The electronic transition to the excited state occurs when the excitation wavelength corresponds to the energy difference between the ground state and the excited state. In the excited state, some energy is lost to vibrational relaxation. As a result, fluorescence energy is less than the absorption energy and the emitted light is observed at a longer wavelength than the excitation wavelength.

Objectives

In this experiment, you will

  • Synthesize fluorescein.
  • Identify the wavelength of maximum absorbance (λ max).
  • Determine the purity of the sample based on Beer’s law.
  • Identify the emission wavelength.

Sensors and Equipment

This experiment features the following Vernier sensors and equipment.

Additional Requirements

You may also need an interface and software for data collection. What do I need for data collection?

Experiment 23 from Organic Chemistry with Vernier Lab Book

<i>Organic Chemistry with Vernier</i> book cover

Included in the Lab Book

Vernier lab books include word-processing files of the student instructions, essential teacher information, suggested answers, sample data and graphs, and more.

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