Vernier Software and Technology
Vernier Software & Technology

Biochemical Oxygen Demand

Figure from experiment 16 from Investigating Environmental Science through Inquiry


Oxygen available to aquatic organisms is found in the form of dissolved oxygen. Oxygen gas is dissolved in a stream through aeration, diffusion from the atmosphere, and photosynthesis of aquatic plants and algae. Plants and animals in the stream consume oxygen in order to produce energy through respiration. In a healthy stream, oxygen is replenished faster than it is used by aquatic organisms. In some streams, aerobic bacteria decompose such a large volume of organic material that oxygen is depleted from the stream faster than it can be replaced. The resulting decrease in dissolved oxygen is known as the Biochemical Oxygen Demand (BOD).

When it rains, organic material found in the soil is transported in the rainwater to streams and rivers. Additional organic material accumulates in the stream when aquatic organisms die. Bacteria and other microorganisms decompose this organic material. In a healthy body of water, this process has only a slight impact on dissolved oxygen levels. It serves to release vital nutrients, such as nitrates and phosphates, which stimulate algae and aquatic plant growth. If the amount of decomposing organic material is too high, dissolved oxygen levels can be severely reduced. In a body of water with large amounts of decaying organic material the dissolved oxygen levels may drop by 90%—this would represent a high BOD. In a mountain stream with low levels of decaying organic material, the dissolved oxygen levels may drop by only 10% or 20%—a low BOD.


In the Preliminary Activity, you will gain experience using a Dissolved Oxygen Probe as you determine the DO level of a water sample provided by your teacher.

After completing the Preliminary Activity, you will first use reference sources to find out more about BOD before you choose and investigate a researchable question dealing with BOD.

Sensors and Equipment

This investigation features the following Vernier sensors and equipment.

Additional Requirements

You may also need an interface and software for data collection. What do I need for data collection?

Standards Correlations

See all standards correlations for Investigating Environmental Science through Inquiry »

Investigation 16 from Investigating Environmental Science through Inquiry Lab Book

<em>Investigating Environmental Science through Inquiry</em> book cover

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