Vernier Software and Technology
Vernier Software & Technology

Dissolved Oxygen


Oxygen gas dissolved in water is vital to the existence of most aquatic organisms. Oxygen is a key component in cellular respiration for both aquatic and terrestrial life. The concentration of dissolved oxygen, DO, in an aquatic environment is an important indicator of the environment’s water quality.

Some organisms, such as salmon, mayflies, and trout, require high concentrations of dissolved oxygen. Other organisms, such as catfish, mosquito larvae, and carp, can survive in environments with lower concentrations of dissolved oxygen. The diversity of organisms is greatest at higher DO concentrations. Table 1 lists the minimum dissolved oxygen concentrations necessary to sustain selected animals.

Table 1: Minimum DO Requirements
Organism Minimum dissolved oxygen (mg/L)
Smallmouth bass6.5
Caddisfly larvae4.0
Mayfly larvae4.0
Mosquito larvae1.0

Oxygen gas is dissolved in water by a variety of processes—diffusion between the atmosphere and water at its surface, aeration as water flows over rocks and other debris, churning of water by waves and wind, and photosynthesis of aquatic plants. There are many factors that affect the concentration of dissolved oxygen in an aquatic environment. These factors include: temperature, stream flow, air pressure, aquatic plants, decaying organic matter, and human activities.

As a result of plant activity, DO levels may fluctuate during the day, rising throughout the morning and reaching a peak in the afternoon. At night photosynthesis ceases, but plants and animals continue to respire, causing a decrease in DO levels. Because large daily fluctuations are possible, DO tests should be performed at the same time each day. Large fluctuations in dissolved oxygen levels over a short period of time may be the result of an algal bloom. While the algae population is growing at a fast rate, dissolved oxygen levels increase. Soon the algae begin to die and are decomposed by aerobic bacteria, which use up the oxygen. As a greater number of algae die, the oxygen requirement of the aerobic decomposers increases, resulting in a sharp drop in dissolved oxygen levels. Following an algal bloom, oxygen levels can be so low that fish and other aquatic organisms can suffocate and die.

Temperature is important to the ability of oxygen to dissolve, because oxygen, like all gases, has different solubilities at different temperatures. Cooler waters have a greater capacity for dissolved oxygen than warmer waters. Human activities, such as the removal of foliage along a stream or the release of warm water used in industrial processes, can cause an increase in water temperature along a given stretch of the stream. This results in a lower dissolved oxygen capacity for the stream.


  • Measure the level of dissolved oxygen in a stream or lake using a Dissolved Oxygen Probe.
  • Determine the percent saturation.

Sensors and Equipment

This experiment features the following Vernier sensors and equipment.

Additional Requirements

You may also need an interface and software for data collection. What do I need for data collection?

Standards Correlations

See all standards correlations for Water Quality with Vernier »

Water Quality with Vernier

See other experiments from the lab book.

4Total Solids
5Dissolved Oxygen
6Biochemical Oxygen Demand
10Ammonium Nitrogen
12Total Dissolved Solids
13Calcium and Water Hardness
14Total Water Hardness
15Chloride and Salinity
16Stream Flow
17Physical Profile of a Lake
18PAR Attenuation in Water

Experiment 5 from Water Quality with Vernier Lab Book

<i>Water Quality with Vernier</i> book cover

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