{"id":1434,"date":"2015-02-06T16:48:31","date_gmt":"2015-02-07T00:48:31","guid":{"rendered":""},"modified":"2025-04-24T09:51:16","modified_gmt":"2025-04-24T16:51:16","slug":"1434","status":"publish","type":"post","link":"https:\/\/www.vernier.com\/til\/1434","title":{"rendered":"Calcium Ion-Selective Electrode Troubleshooting and FAQs"},"content":{"rendered":"<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:60%\">\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<h3 class=\"wp-block-heading\"><a href=\"\/product\/calcium-ion-selective-electrode\/\">Calcium Ion-Selective Electrode<\/a> (<nobr>CA-BTA<\/nobr>)<\/h3>\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<p><a href=\"\/manuals\/CA-BTA\">Specifications and User Guide<\/a><\/p>\n<\/div>\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:40%\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/www.vernier.com\/wp-content\/uploads\/2019\/11\/product.ca-bta._hero.001-scaled.jpg\" alt=\"\"\/><\/figure>\n<\/div>\n<\/div>\n<h3 class=\"wp-block-heading\">Troubleshooting<\/h3>\n<ul class=\"wp-block-list\">\n<li><strong>Primary Test:<\/strong> Check the raw voltages while calibrating in the standards. The high (1000 mg\/L) should be around 1.9 volts. The low (10 mg\/L) should be around 1.5 volts.<\/li>\n<li><strong>Secondary Test:<\/strong> Refer to <a href=\"\/til\/665\/\">General tips for using Ion Selective Electrodes (ISE).<\/a><\/li>\n<\/ul>\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube.com\/embed\/sgXm9vuO8Zk\" width=\"560\" height=\"315\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<h3 class=\"wp-block-heading\">Additional Troubleshooting<\/h3>\n<ul class=\"wp-block-list\">\n<li><a href=\"\/til\/1060\/\">Do any of your sensors require special storage?<\/a><\/li>\n<li><a href=\"\/til\/759\/\">Do you sell ISE Standards?<\/a><\/li>\n<li><a href=\"\/til\/665\/\">General tips for using Ion Selective Electrodes (ISE).<\/a><\/li>\n<li><a href=\"\/til\/1296\/\">How can I have my ISE read mV output instead of mg\/L?<\/a><\/li>\n<li><a href=\"\/til\/1463\/\">What is the Difference Between Auto-ID Sensors, Non-Auto-ID Sensors, Smart Sensors, and Go Direct Sensors?<\/a><\/li>\n<li><a href=\"\/til\/3389\/\">How do I sterilize an Ion-Selective Electrode?<\/a><\/li>\n<\/ul>\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<h3 class=\"wp-block-heading\">Specifications<\/h3>\n<ul class=\"wp-block-list\">\n<li>Range (concentration): 1 to 40,000 mg\/L (or ppm)<\/li>\n<li>Reproducibility (precision): \u00b1 10% of full scale (calibrated 10 to 1000 mg)<\/li>\n<li>Interfering ions: Pb<sup>2+<\/sup>, Hg<sup>2+<\/sup>, Si<sup>2+<\/sup>, Fe<sup>2+<\/sup>, Cu<sup>2+<\/sup>, Ni<sup>2+<\/sup>, NH<sub>3<\/sub>, Na<sup>+<\/sup>, Tris<sup>+<\/sup>, Li<sup>+<\/sup>, K<sup>+<\/sup>, Ba<sup>2+<\/sup>, Zn<sup>2+<\/sup>, Mg<sup>2+<\/sup><\/li>\n<li>pH range: 3 to 10  (no pH compensation)<\/li>\n<li>Temperature range: 0 to 40 \u00b0C (no temperature compensation)<\/li>\n<li>Electrode slope: +26 \u00b12 mV\/decade at 25\u00b0C<\/li>\n<li>Approximate calibration voltages: <br \/>\u26ac High (1000 mg\/L): 1.9 V<br \/>\u26ac Low (10 mg\/L): 1.5 V<\/li>\n<li>Electrode Resistance: 100 M\u03a9<\/li>\n<li>Minimum immersion: 2.8 cm (1.1 inch)<\/li>\n<li>Probe Dimensions<br \/>\u26ac Electrode length: 155 mm<br \/>\u26ac Body diameter: 12 mm<br \/>\u26ac Cap diameter: 16 mm<br \/>\u26ac Cable length: 100 cm<\/li>\n<\/ul>\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<h3 class=\"wp-block-heading\">Calibration<\/h3>\n<p>Calibrate? Yes. Conduct a two-point calibration, using the standard solutions that are shipped with the sensor. For more information on calibration, see <a href=\"\/til\/4011\/\">How do I calibrate my sensor?<\/a><\/p>\n<ol class=\"wp-block-list\">\n<li><strong>First point<\/strong>\n<ol style=\"list-style-type:lower-alpha\" class=\"wp-block-list\">\n<li>The ISE should be soaking in the High Standard. <\/li>\n<li>Enter the concentration value of the High Standard, then click or tap Keep.<\/li>\n<\/ol>\n<\/li>\n<li><strong>Second point<\/strong>\n<ol style=\"list-style-type:lower-alpha\" class=\"wp-block-list\">\n<li>Remove the ISE from the High Standard.<\/li>\n<li>Rinse the electrode well with distilled water from a wash bottle.<\/li>\n<li>Gently blot dry with a paper towel or lab wipe. <\/li>\n<li>Place the electrode into the Low Standard. Wait for the voltage reading to stabilize.<\/li>\n<li>Enter the concentration value of the Low Standard, then click or tap Keep.<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<p><strong>Important:<\/strong> Make sure the ISE is not resting on the bottom of the container and that the small white reference contacts are immersed. Make sure no air bubbles are trapped below the ISE. Hold the ISE still and wait for the voltage reading to stabilize. Stirring the solution during this proc<\/p>\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h3 class=\"wp-block-heading\">Related Products<\/h3>\n<ul class=\"wp-block-list\">\n<li><a href=\"\/product\/electrode-support\/\">Electrode Support<\/a> (<nobr>ESUP<\/nobr>)<\/li>\n<li><a href=\"\/product\/go-direct-calcium-ion-selective-electrode\/\">Go Direct<sup>&reg;<\/sup> Calcium Ion-Selective Electrode<\/a> (<nobr>GDX-CA<\/nobr>)<\/li>\n<\/ul>\n<\/div>\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<h3 class=\"wp-block-heading\">Replacement Parts<\/h3>\n<ul class=\"wp-block-list\">\n<li><a href=\"\/product\/calcium-replacement-module\/\">Calcium Replacement Module<\/a> (<nobr>CA-MOD<\/nobr>)<\/li>\n<li><a href=\"\/product\/standard-ca-ise-solution\/\">Standard Ca ISE Solution<\/a> (<nobr>CA-LST<\/nobr>)\u2014Low Concentration<\/li>\n<li><a href=\"\/product\/standard-ca-ise-solution\/\">Standard Ca ISE Solution<\/a> (<nobr>CA-HST<\/nobr>)\u2014High Concentration<\/li>\n<li><a href=\"\/product\/electrode-storage-bottles\/\">Electrode Storage Bottles<\/a> (<nobr>BTL-ES<\/nobr>)<\/li>\n<\/ul>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Specifications and User Guide Troubleshooting Additional Troubleshooting Specifications Calibration Calibrate? Yes. Conduct a two-point calibration, using the standard solutions that are shipped with the sensor&#8230;.<\/p>\n","protected":false},"author":1073,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[622,1889,1890,1891,1883,720],"class_list":["post-1434","post","type-post","status-publish","format-standard","hentry","tag-ca-bta","tag-caise","tag-calciummain","tag-camain","tag-isemain","tag-maintil"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/posts\/1434","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/users\/1073"}],"replies":[{"embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/comments?post=1434"}],"version-history":[{"count":0,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/posts\/1434\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/media?parent=1434"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/categories?post=1434"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/tags?post=1434"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}