{"id":1683,"date":"2019-05-20T21:10:39","date_gmt":"2019-05-21T04:10:39","guid":{"rendered":""},"modified":"2024-07-18T14:04:19","modified_gmt":"2024-07-18T21:04:19","slug":"1683","status":"publish","type":"post","link":"https:\/\/www.vernier.com\/til\/1683","title":{"rendered":"Do you have a spectrometer that can measure DNA concentration and purity?"},"content":{"rendered":"<p>Yes, the following Vernier spectrometers can be used to assess DNA purity and concentration:<\/p>\n<p><a href=\"\/product\/go-direct-uv-vis-spectrophotometer\/\">Go Direct<sup>&reg;<\/sup> UV-VIS Spectrophotometer<\/a> (<nobr>GDX-SPEC-UV<\/nobr>)<br \/>\n<a href=\"\/product\/vernier-uv-vis-spectrophotometer\/\">Vernier UV-VIS Spectrophotometer<\/a> (<nobr>VSP-UV<\/nobr>)<br \/>\n<a href=\"\/product\/go-direct-fluorescence-uv-vis-spectrophotometer\/\">Go Direct<sup>&reg;<\/sup> Fluorescence\/UV-VIS Spectrophotometer<\/a> (<nobr>GDX-SPEC-FUV<\/nobr>)<br \/>\n<a href=\"\/product\/vernier-fluorescence-uv-vis-spectrophotometer\/\">Vernier Fluorescence\/UV-VIS Spectrophotometer<\/a> (<nobr>VSP-FUV<\/nobr>)<\/p>\n<p>DNA has an absorption maxima at approximately 260nm. At 260nm, an A260 value of 1.0 is set for a pure solution of dsDNA with a concentration of 50 ug\/mL.<\/p>\n<p>We have a free downloadable experiment on Nucleic Acid Quantitation here:<\/p>\n<p><a href=\"https:\/\/turbo.vernier.com\/wp-content\/uploads\/2022\/01\/nucleic-acids.zip\">Nucleic Acid Quantitation<\/a><\/p>\n<p>See also: <a href=\"\/til\/2578\/\">How do I get an A260\/A280 value with your UV-VIS spectrometers in Logger <i>Pro<\/i>?<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p>Yes, the following Vernier spectrometers can be used to assess DNA purity and concentration: DNA has an absorption maxima at approximately 260nm. At 260nm, an&#8230;<\/p>\n","protected":false},"author":11015,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[2530,2529,2526,2527,2528],"class_list":["post-1683","post","type-post","status-publish","format-standard","hentry","tag-dna","tag-a260-a280-ratio","tag-dna-concentration","tag-rna-concentration","tag-uv-vis"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/posts\/1683","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/users\/11015"}],"replies":[{"embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/comments?post=1683"}],"version-history":[{"count":0,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/posts\/1683\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/media?parent=1683"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/categories?post=1683"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/tags?post=1683"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}