{"id":3044,"date":"2019-05-20T21:29:03","date_gmt":"2019-05-21T04:29:03","guid":{"rendered":""},"modified":"2023-04-15T12:48:07","modified_gmt":"2023-04-15T19:48:07","slug":"3044","status":"publish","type":"post","link":"https:\/\/www.vernier.com\/til\/3044","title":{"rendered":"UV-VIS Spectrophotometer Troubleshooting and FAQs"},"content":{"rendered":"

Go Direct UV-VIS Spectrophotometer<\/a>
\nFor general instructions, see Specifications and User Guide
\n<\/a>
\nVernier UV-VIS Spectrophotometer<\/a>
\nFor general instructions, see Specifications and User Guide<\/a><\/p>\n

TROUBLESHOOTING<\/p>\n

Primary Test: Are you using LabQuest App v2.8.8 or v3.0.5, or Spectral Analysis v4.11, Logger Pro<\/i> 3.16.1 or newer?<\/p>\n

    \n
  1. Connect the AC power supply to the spectrophotometer. Turn the power switch to the ON position. Wait for the lamp indicator LED to remain green.<\/li>\n
  2. If connecting via USB: Connect the spectrophotometer directly to the USB port. If connecting via bluetooth (Go Direct version only): Connect the spectrometer to the USB power adapter or to a powered USB hub. The bluetooth LED will blink. Note: Do NOT plug the USB into a computer USB port to run with Bluetooth.<\/li>\n
  3. Start the appropriate data-collection software. Options include: Logger Pro<\/em> 3, LabQuest App, and Spectral Analysis. The latest version of software is available as a free download here: https:\/\/www.vernier.com\/downloads\/<\/a><\/li>\n
  4. Set the spectrometer to collect data in absorbance vs wavelength mode, if not already done.<\/li>\n
  5. Calibrate the spectrometer with the appropriate solvent.<\/li>\n
  6. Collect a spectrum with a known sample where the absorbance falls between 0.1 and 1.0 absorbance units.<\/li>\n<\/ol>\n

    RELATED TIL ENTRIES
    Most Common:
    My Spectrometer is not working properly. It won't calibrate or is giving very noisy data.<\/a>
    What is the difference between all your spectrometers?<\/a>
    What cuvettes can I use in my UV-Vis or Fluorescence\/UV-VIS Spectrophotometer?<\/a>
    What is the Z dimension or beam height of your Spectrometers?<\/a>
    Why are there blank data table cells in my spectrometer absorbance data?<\/a>
    Why is the absorbance reading on my device (spectrometer\/colorimeter) unstable or nonlinear at values above 1.0?<\/a><\/p>\n

    General:
    What is the difference between the types of cuvettes you sell for colorimeters and spectrophotometers?<\/a>
    Does the Vernier Spectrophotometer Optical Fiber work with any of the Ocean Optics Spectrometers or vice versa?<\/a>
    What cuvettes can I use in my UV-Vis or Fluorescence\/UV-VIS Spectrophotometer?<\/a>
    What is the beam height for the Vernier UV-VIS Spectrophotometer?<\/a>
    What is the Z dimension or beam height of your Spectrometers?<\/a>
    The power LED indicator light on my UV-VIS or Fluorescence\/UV-VIS Spectrometer is red.<\/a><\/p>\n

    Software Issues:
    Why is the maximum sample time for a spectrometer set at 1000 milliseconds?<\/a>
    Why are there blank data table cells in my spectrometer absorbance data?<\/a>
    Spectrometer Error: 'Could Not Collect Values' or 'Calibration Failed'<\/a>
    Is there a way to toggle on\/off the colored background behind the absorbance spectrum graph?<\/a>
    My Spectrometer is not working properly. It won't calibrate or is giving very noisy data.<\/a>
    Why can't my device find Go Direct Spectrometer when I try to connect via Bluetooth?<\/a><\/p>\n

    Experiments:
    Do you have labs written for your spectrophotometers?<\/a>
    Can I do blackbody experiments with your Spectrometers?<\/a>
    Do you have a spectrometer that can measure DNA concentration and purity?<\/a>
    Can I use my spectrometer to collect data at one wavelength (Beer's law or kinetics)?<\/a>
    How do I get an A260\/A280 value with your UV-VIS spectrometers in Logger Pro<\/i>?<\/a>
    Why is the absorbance reading on my device (spectrometer\/colorimeter) unstable or nonlinear at values above 1.0?<\/a><\/p>\n

    SPECIFICATIONS<\/a><\/p>\n

    CALIBRATION
    You must calibrate the spectrometer every time you use Absorbance or %T mode. Calibration is not required for intensity.<\/p>\n

    RELATED VERNIER PRODUCTS
    Go Direct®<\/sup> Fluorescence\/UV-VIS Spectrophotometer<\/a> (GDX-SPEC-FUV<\/nobr>)
    Go Direct®<\/sup> SpectroVis®<\/sup> Plus Spectrophotometer<\/a> (GDX-SVISPL<\/nobr>)
    Go Direct®<\/sup> Visible Spectrophotometer<\/a> (GDX-SPEC-VIS<\/nobr>)
    Replacement Power Supply for UV-VIS and Fluorescence\/UV-VIS Spectrophotometer<\/a> (VSP-UV-PS<\/nobr>)
    Quartz Cuvettes<\/a> (CUV-QUARTZ<\/nobr>)
    Plastic Cuvettes (UV-VIS)<\/a> (CUV-UV<\/nobr>)<\/p>","protected":false},"excerpt":{"rendered":"

    Go Direct UV-VIS Spectrophotometer For general instructions, see Specifications and User Guide Vernier UV-VIS Spectrophotometer For general instructions, see Specifications and User Guide TROUBLESHOOTING Primary…<\/p>\n","protected":false},"author":11015,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[],"tags":[1386,2174,2163,5128,5129,5127,5126],"class_list":["post-3044","post","type-post","status-publish","format-standard","hentry","tag-spec","tag-specmain","tag-spectrometer","tag-uv-uvvismain-uv-vismain","tag-uvmain","tag-vernier-uv-vis","tag-vspuvmain"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/posts\/3044","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/users\/11015"}],"replies":[{"embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/comments?post=3044"}],"version-history":[{"count":0,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/posts\/3044\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/media?parent=3044"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/categories?post=3044"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.vernier.com\/til\/wp-json\/wp\/v2\/tags?post=3044"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}