-Primary Test: Connect an ion selective electrode to the amplifier and connect the amplifier to your platform. Start the data-collection program. Place the electrode in a standard solution of known value and confirm that the readings are within range.
-Secondary Test: Confirm that the BNC connection is secure.
-Calibrate? It depends on the electrode that is connected. If you have an electrode connected to read Voltage, calibration is disabled. If you have an electrode connected to read ion concentration, you may perform a two or three point calibration in Graphical Analysis 4 software.
See TIL 4011: How do I calibrate my sensor?