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UV-VIS Spectrophotometer Troubleshooting and FAQs

  • May 20, 2019
  • 0
Go Direct UV-VIS Spectrophotometer For general instructions, see Specifications and User Guide Vernier UV-VIS Spectrophotometer For general instructions, see Specifications and User Guide TROUBLESHOOTING Primary Test: Are you using LabQuest A...
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How do I get an A260/A280 value with your UV-VIS spectrometers in Logger Pro?

  • May 20, 2019
  • 0
WHAT IS THE A260/A280 RATIO? This ratio is used to determine the purity of a DNA or protein sample. Contamination of a nucleic acid solution by proteins, carbohydrates, and other organic molecules can be determined using a procedure called the A2...
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Do you have a spectrometer that can measure DNA concentration and purity?

  • May 20, 2019
  • 0
Yes, the following Vernier spectrometers can be used to assess DNA purity and concentration: Go Direct® UV-VIS Spectrophotometer (GDX-SPEC-UV) Vernier UV-VIS Spectrophotometer (VSP-UV) Go Direct® Fluorescence/UV-VIS Spectrophotometer (GDX-SP...
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Can I use my spectrometer to collect data at one wavelength (Beer’s law or kinetics)?

  • May 20, 2019
  • 0
A full spectrum is taken in order to identify the wavelength for analysis. If you already know the wavelength for analysis, then a full spectrum is not necessary. To set up data collection for modes other than full spectrum, follow the instructions ...
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Do you have labs written for your spectrophotometers?

  • May 20, 2019
  • 0
We have posted several Spectrometer lab experiments on our website for all of our spectrometers. To find the labs, go to the individual spectrometer's webpage and click on the "Experiments" tab. Go Direct® SpectroVis® Plus Spectrophotometer ...
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My Spectrometer is not working properly. It won’t calibrate or is giving very noisy data.

  • May 20, 2019
  • 0
If your spectrometer isn’t calibrating or is showing unusually noisy or high absorbance values (often above 3 or blank), the issue may be due to insufficient light reaching the detector. This happens when: The light source is weak in a certain...
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Why are there blank data table cells in my spectrometer absorbance data?

  • May 20, 2019
  • 0
Sometimes the counts from the light striking a pixel is not sufficiently different from the counts when the pixel is in the dark to make a reliable absorbance reading. This can happen either when the light source is weak in that spectral region, or w...
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How is the temperature compensation calculated on my conductivity probe (CON-BTA)?

  • May 17, 2019
  • 0
The Conductivity Probe (CON-BTA) is automatically temperature compensated between temperatures of 5 and 35°C. Note that the temperature of a solution is being read by a thermistor that extends into the space between the graphite electrodes. Readings...
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How can I use Vernier sensors to measure sports activities?

  • May 16, 2019
  • 0
Below are some of the sports-related activities we have done using Vernier sensors. Using a Goniometer to Explore Heart Rate and Cadencehttps://www.vernier.com/vernier-ideas/using-a-goniometer-to-explore-heart-rate-and-cadence/ Analysis of Bar...
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Are your sensors compatible with Google Science Journal?

  • May 14, 2019
  • 0
Yes. Go Wireless and some Go Direct sensors can be used with Google Science Journal. For more information on Science Journal, see https://www.vernier.com/products/software/science-journal/ https://makingscience.withgoogle.com/science-journa...
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