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3.5 Genetic Modification and Biotechnology

Experiments

Here are experiments our science specialists have selected to support the IB* topic.

Recommended

pGLO™ Bacterial Transformation

Experiment #6A from Advanced Biology with Vernier

In this experiment, you will

  • Use a plasmid vector to transform bacteria with genes for Green Fluorescent Protein (GFP) and antibiotic resistance in a controlled experiment.
  • Use the heat shock method of transforming E. coli.
  • Regulate the expression of the GFP gene using arabinose.
  • Describe the biological process involved in transforming bacterial cells.
  • Calculate your transformation efficiency.
  • Learn basic molecular biology techniques.

Recommended

Forensic DNA Fingerprinting

Experiment #6B from Advanced Biology with Vernier

In this activity, you will

  • Digest DNA found at the “crime scene” and the DNA of five “suspects” with two restriction enzymes.
  • Perform agarose gel electrophoresis on DNA samples.
  • Stain the gel to visualize the DNA bands.
  • Document and examine gel results with an imaging system.
  • Evaluate who cannot be excluded from the investigation by constructing a standard curve and determining the size of the DNA fragments from the gel using Logger Pro.

Analysis of Precut Lambda DNA

Experiment #6B from Advanced Biology with Vernier

In this experiment, you will

  • Perform agarose gel electrophoresis using three different predigested samples of lambda DNA and uncut lambda DNA.
  • Stain the gel.
  • Document and examine gel results with an imaging system.
  • Construct a standard curve and determine the size of the DNA fragments from the gel using Logger Pro.

Educational Standard
International Baccalaureate (IB)
Subject
Biology
Section
Core
Topic
3. Genetics

* The IB Diploma Program is an official program of the International Baccalaureate Organization (IBO) which authorizes schools to offer it. The material available here has been developed independently of the IBO and is not endorsed by it.

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