Recommended

pGLO™ Bacterial Transformation

Experiment #6A from Advanced Biology with Vernier

In this experiment, you will

• Use a plasmid vector to transform bacteria with genes for Green Fluorescent Protein (GFP) and antibiotic resistance in a controlled experiment.
• Use the heat shock method of transforming E. coli.
• Regulate the expression of the GFP gene using arabinose.
• Describe the biological process involved in transforming bacterial cells.
• Calculate your transformation efficiency.
• Learn basic molecular biology techniques.

Recommended

Forensic DNA Fingerprinting

Experiment #6B from Advanced Biology with Vernier

In this activity, you will

• Digest DNA found at the “crime scene” and the DNA of five “suspects” with two restriction enzymes.
• Perform agarose gel electrophoresis on DNA samples.
• Stain the gel to visualize the DNA bands.
• Document and examine gel results with an imaging system.
• Evaluate who cannot be excluded from the investigation by constructing a standard curve and determining the size of the DNA fragments from the gel using Logger Pro.

Analysis of Precut Lambda DNA

Experiment #6B from Advanced Biology with Vernier

In this experiment, you will

• Perform agarose gel electrophoresis using three different predigested samples of lambda DNA and uncut lambda DNA.
• Stain the gel.
• Document and examine gel results with an imaging system.
• Construct a standard curve and determine the size of the DNA fragments from the gel using Logger Pro.