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A1.2.9—Diversity of possible DNA base sequences and the limitless capacity of DNA for storing information

Experiments

Here are experiments our science specialists have selected to support this IB* topic.

Analysis of Precut Lambda DNA

Experiment #6B from Advanced Biology with Vernier

In this experiment, you will

  • Perform agarose gel electrophoresis using three different predigested samples of lambda DNA and uncut lambda DNA.
  • Stain the gel.
  • Document and examine gel results with an imaging system.
  • Construct a standard curve and determine the size of the DNA fragments from the gel using Logger Pro.

Forensic DNA Fingerprinting

Experiment #6B from Advanced Biology with Vernier

In this activity, you will

  • Digest DNA found at the “crime scene” and the DNA of five “suspects” with two restriction enzymes.
  • Perform agarose gel electrophoresis on DNA samples.
  • Stain the gel to visualize the DNA bands.
  • Document and examine gel results with an imaging system.
  • Evaluate who cannot be excluded from the investigation by constructing a standard curve and determining the size of the DNA fragments from the gel using Logger Pro.

Educational Standard
International Baccalaureate (IB) 2025
Subject
Biology
Theme
A Unity and Diversity
Level of organization
1 Molecules
Topic
A1.2 Nucleic acids
Section
Standard level and higher level

* The IB Diploma Program is an official program of the International Baccalaureate Organization (IBO) which authorizes schools to offer it. The material available here has been developed independently of the IBO and is not endorsed by it.

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