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B.9 Biological pigments (Higher Level)

Experiments

Here are experiments our science specialists have selected to support the IB* topic.

Extraction of Spinach Pigments and Analysis by Electronic Absorption Spectroscopy

Experiment #12 from Organic Chemistry with Vernier

In this experiment, you will

  • Extract the pigments in spinach leaves.
  • Determine the Rf values of the pigments in spinach.
  • Isolate the green and yellow pigments using column chromatography.
  • Identify the wavelengths of absorbance peaks for the extracted spinach pigments.

Educational Standard
International Baccalaureate (IB)
Subject
Chemistry
Section
Options
Topic
B. Biochemistry

* The IB Diploma Program is an official program of the International Baccalaureate Organization (IBO) which authorizes schools to offer it. The material available here has been developed independently of the IBO and is not endorsed by it.

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