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Experiments​

Sugar Metabolism with Yeast (Carbon Dioxide Gas)

Experiment #10A from Investigating Biology through Inquiry

Education Level
High School
College
Subject
Biology

Introduction

Yeast are able to metabolize some foods, but not others. In order for an organism to make use of a potential source of food, it must be capable of transporting the food into its cells. It must also have the proper enzymes capable of breaking the food’s chemical bonds in a useful way. Sugars are vital to all living organisms. Yeast are capable of using some, but not all sugars as a food source. Yeast can metabolize sugar in two ways, aerobically, with the aid of oxygen, or anaerobically, without oxygen. In both cases, carbon dioxide, CO2, is produced. The rate that this gas is produced is referred to as the rate of respiration.

Objectives

In the Preliminary Activity, you will use a CO2 Gas Sensor to determine the rate of respiration of glucose by yeast.

After completing the Preliminary Activity, you will first use reference sources to find out more about sugars, respiration, and yeast before you choose and investigate a researchable question dealing with the respiration of sugars by yeast. Some topics to consider in your reference search are:

  • sugars
  • carbohydrates
  • monosaccharides
  • disaccharides
  • polysaccharides
  • yeast
  • glycolysis
  • metabolism
  • aerobic respiration
  • anaerobic respiration

Sensors and Equipment

This experiment features the following sensors and equipment. Additional equipment may be required.

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This experiment is #10A of Investigating Biology through Inquiry. The experiment in the book includes student instructions as well as instructor information for set up, helpful hints, and sample graphs and data.

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