O2 Gas Sensor (O2-BTA)
Specifications and User Guide

Primary Test: Blow on the sensor. Do you see a decrease in the Oxygen Gas reading? This tells you that the sensor is responding.

Things to Consider:
1. Has the sensor been stored upright? The O2 Gas Sensor must be stored upright when not being used. This is necessary to maintain the sensor. Failure to store upright will reduce the life of the sensor.

2. Is your data-collection period too short? Diffusion of gases is a fairly slow process; there can be some delay in readings.

3. Are you collecting data in a controlled environment? To collect data in a controlled environment, use the 250 mL Nalgene collection bottle that is included with the sensor.

How to calibrate the O2 Gas Sensor (O2-BTA)
Do any of your sensors require special storage?
Can my O2 Gas Sensor (O2-BTA) be repaired?
Which Oxygen Gas Sensor (O2-BTA) do I have?
Can I use a dishwasher to wash the Nalgene bottle that shipped with my O2 or CO2 Gas Sensor?
How can I insert a CO2 Gas Sensor or O2 Gas Sensor into my own container?
Can O2 gas concentration be expressed in units of mg/m³?
What is the volume of gas displaced by the shaft of the CO2 and O2 Gas Sensor? What is the total volume of the Large and Small BioChambers?

-Measurement Range: 0–27% (0-270 ppt)
-Accuracy (@ Standard Pressure 760 mm Hg): +/- 1% volume O2
-Typical Resolution: 0.01%
-Response Time: 90% of final reading in ~12 seconds
-Warm-Up Time: Less than 5 seconds
-Pressure Effect: Directly proportional
-Vout = Vout (standard) x (P/1013)
-Pressure range: 0.5 atm to 1.5 atm
-Output Signal Range: 0.3 to 5 VDC; 2.7 to 3.8 VDC @ 21% O2
-Output Impedance: 1 KΩ
-Input Voltage: 5 VDC +/–0.25 VDC
-Gas Sampling Mode: Diffusion
-Normal Operating Temperature Range: 20°C to 30°C
-Operating Temperature Range: 5 to 40°C (with calibration)
-Operating Humidity Range: 0 to 95% RH
-Storage Temperature Range: –20 to +60°C
-Dimensions: Sensor Tube: 45 mm length, 28 mm OD
– Total length: 135 mm

-The sensor will not calibrate initially, but will drift over time.
-If you wish to calibrate the sensor, follow the normal 2-point calibration procedure. For the first point, push and hold the calibrate button on the sensor using a paper clip. Enter a value of 0 for this reading. Release the button and take a second reading in air. Enter a value of 20.9% oxygen or a corrected value from the table in the sensor booklet.


Using your LabQuest 2 stylus or a paper clip, you can easily calibrate your O₂ Gas Sensor. #VernierTechTips

A video posted by Vernier Software & Technology (@verniersoftware) on

250 mL Nalgene Bottle w/Lid (CO2-BTL)
BioChamber 250 (BC-250)
BioChamber 2000 (BC-2000)