Vernier Software and Technology
Vernier Software & Technology
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Vernier Fluorescence/UV-VIS Spectrophotometer Troubleshooting and FAQs

Vernier Fluorescence/UV-VIS Spectrophotometer, order code VSP-FUV, $2,899
For general instructions, see Specifications and User Guide

TROUBLESHOOTING
- Primary Test:
Are you using Spectral Analysis 4.8 or newer? LabQuest 2.7.2 or newer? Logger Pro 3.14 or newer?

TIPS:
1. If you plan on only taking fluorescence data, the power cable does not need to be attached, nor does the power switch need to be on. This will help save your deuterium lamp for absorbance measurements.
2. In fluorescence measurements, the inner-filter effect is always a consideration. The inner filter effect results in an apparent decrease in emission quantum yield and/or distortion of bandshape as a result of reabsorption of emitted radiation. To avoid this, it is best to perform fluorescence measurements on samples that have an absorbance below 0.1.

RELATED TIL ENTRIES
General:
TIL 4198: Tips for improving your Vernier Fluorescence/UV-VIS Spectrophotometer data
TIL 4063: Can I order a custom LED for the Fluorescence/UV-VIS Spectrometer (VSP-FUV)?
TIL 2016: What is the difference between the types of cuvettes you sell for Spectrometers and the Colorimeter?
TIL 3194: What cuvettes can I use in the Vernier UV-Vis or Fluorescence/UV-VIS Spectrophotometer?
TIL 2416: How do I check the lamp output of my spectrometer?
TIL 2571: Can my Vernier-branded spectrometer be reconditioned? Can the lamp in my spectrometer be replaced?

Software Issues:
TIL 2066: Why is the maximum sample time for a spectrometer set at 1000 milliseconds?
TIL 1850: Why are there blank data table cells in my spectrometer absorbance data?
TIL 2408: My spectrometer is connected to a computer and I get the error message, "Could not collect values from device," during calibration.
TIL 2853: Is there a way to toggle on/off the colored background behind the absorbance spectrum graph?
TIL 2354: My UV-VIS Spectrometer is not working properly. It won't calibrate or it is not reporting across the proper wavelength range?
TIL 3885: Spectral Analysis 4 Troubleshooting and FAQs
TIL 3957: The LED indicator light on my Vernier UV-VIS Spectrometer (VSP-UV) is red.

Experiments:
TIL 1577: Do you have labs written for your spectrophotometers?
TIL 1563: Can I do blackbody experiments with the Vernier or Ocean Optics Spectrometers?
TIL 1683: Do you have a spectrometer that can measure DNA concentration and purity?
TIL 2404: Can I use my spectrometer to collect data at one wavelength (kinetics or Beer's law)?
TIL 2578: How do I get an A260/A280 value with your UV-VIS spectrometers in Logger Pro?
TIL 2589: Why is the absorbance reading on my device (spectrometer/colorimeter) unstable or nonlinear at values above 1.0?
TIL 2569: Why don't all of my spectrometers and/or Colorimeters read the same absorbance value for the same sample?

SPECIFICATIONS
TIL 3707: What is the Z dimension or beam height of your Spectrometers?
TIL 2046: What is the path length for the Vernier-branded spectrometers?
Detection modes: Absorbance and Fluorescence
Dimensions: 18.5 cm × 17.0 cm × 7.0 cm
Power supply: AC adapter (included)
Power consumption: 3 A start-up, 500 mA continuous
Absorbance light source: deuterium (UV) and incandescent (VIS)
Emission light source: Exchangeable LED (ships with 375 nm, 450 nm, 525 nm)
Detector: Linear CCD
Wavelength range: 220 – 850 nm
Wavelength reporting interval: ~ 1 nm
Optical resolution: 3.0 nm (as determined with 486 nm hydrogen emission spectral line FWHM)
Wavelength accuracy: ± 2.0 nm (as determined with holmium oxide NIST standard)
Absorbance photometric accuracy: ± 0.05 (as determined with potassium dichromate NIST standards)
Absorbance photometric range (for best accuracy): 0.1 – 1.0
Typical scan time: ~ 2 s
Sample format: 10 mm × 10 mm cuvette (UV fluorescence cuvette included)
Fluorescence emission detection limit: 1 mg/L quinine sulfate dihydrate in 0.1 M H2SO4

CALIBRATION
-Calibrate...
     in Absorbance mode? Yes
     in Transmittance mode? Yes
     in Emission mode? Optional
     in Fluorescence mode? Optional

RELATED VERNIER PRODUCTS
Vernier UV-VIS Spectrophotometer, order code VSP-UV, $2,100
Vernier Spectrometer, order code V-SPEC, $1,799
Vernier Spectrophotometer Optical Fiber, order code VSP-FIBER, $74

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