For general instructions, see Specifications and User Guide
– Primary Test:
Are you using Spectral Analysis 4.8 or newer? LabQuest App 2.7.2 or newer? Logger Pro 3.14 or newer?
– Secondary Test:
Review What are some tips for improving my Vernier Fluorescence/UV-VIS Spectrophotometer data?
- If you plan on only taking fluorescence data, the power cable does not need to be attached, nor does the power switch need to be on. This will help save your deuterium lamp for absorbance measurements.
- In fluorescence measurements, the inner-filter effect is always a consideration. The inner filter effect results in an apparent decrease in emission quantum yield and/or distortion of bandshape as a result of reabsorption of emitted radiation. To avoid this, it is best to perform fluorescence measurements on samples that have an absorbance below 0.1.
RELATED TIL ENTRIES
What are some tips for improving my Vernier Fluorescence/UV-VIS Spectrophotometer data?
What cuvettes can I use in the Vernier UV-Vis or Fluorescence/UV-VIS Spectrophotometer?
Can I order a custom LED for the Fluorescence/UV-VIS Spectrometer (VSP-FUV)?
What is the difference between the types of cuvettes you sell for Spectrometers and the Colorimeter?
How do I check the lamp output of my spectrometer?
Can my Vernier-branded spectrometer be reconditioned? Can the lamp in my spectrometer be replaced?
Why is the maximum sample time for a spectrometer set at 1000 milliseconds?
Why are there blank data table cells in my spectrometer absorbance data?
My spectrometer is connected to a computer and I get the error message, "Could not collect values from device," during calibration.
Is there a way to toggle on/off the colored background behind the absorbance spectrum graph?
My UV-VIS Spectrometer is not working properly. It won't calibrate or it is not reporting across the proper wavelength range?
Spectral Analysis Troubleshooting and FAQs
The LED indicator light on my Vernier UV-VIS Spectrometer (VSP-UV) is red.
Do you have labs written for your spectrophotometers?
Can I do blackbody experiments with the Vernier or Ocean Optics Spectrometers?
Do you have a spectrometer that can measure DNA concentration and purity?
Can I use my spectrometer to collect data at one wavelength (kinetics or Beer's law)?
How do I get an A260/A280 value with your UV-VIS spectrometers in Logger Pro?
Why is the absorbance reading on my device (spectrometer/colorimeter) unstable or nonlinear at values above 1.0?
Why don't all of my spectrometers and/or Colorimeters read the same absorbance value for the same sample?
Tips for measuring Tryptophan fluorescence
What is the Z dimension or beam height of your Spectrometers?
What is the path length for the Vernier-branded spectrometers?
Detection modes: Absorbance and Fluorescence
Dimensions: 18.5 cm × 17.0 cm × 7.0 cm
Power supply: AC adapter (included)
Power consumption: 3 A start-up, 500 mA continuous
Absorbance light source: deuterium (UV) and incandescent (VIS)
Emission light source: Exchangeable LED (ships with 375 nm, 450 nm, 525 nm)
Detector: Linear CCD
Wavelength range: 220 – 850 nm
Wavelength reporting interval: ~ 1 nm
Optical resolution: 3.0 nm (as determined with 486 nm hydrogen emission spectral line FWHM)
Wavelength accuracy: ± 2.0 nm (as determined with holmium oxide NIST standard)
Absorbance photometric accuracy: ± 0.05 (as determined with potassium dichromate NIST standards)
Absorbance photometric range (for best accuracy): 0.1 – 1.0
Typical scan time: ~ 2 s
Sample format: 10 mm × 10 mm cuvette (UV fluorescence cuvette included)
Fluorescence emission detection limit: 1 mg/L quinine sulfate dihydrate in 0.1 M H2SO4
in Absorbance mode? Yes
in Transmittance mode? Yes
in Emission mode? Optional
in Fluorescence mode? Optional