Vernier Software and Technology
Vernier Software & Technology
Tech Info Library

Vernier UV-VIS Spectrophotometer Troubleshooting and FAQs

Vernier UV-VIS Spectrophotometer, order code VSP-UV, $2,100
For general instructions, see Specifications and User Guide

TROUBLESHOOTING
- Primary Test:
1. Connect the AC power supply to the spectrophotometer. Turn the power switch to the ON position. Wait for the lamp indicator LED to remain green.
2. Connect the spectrophotometer directly to the USB port.
3. Start the appropriate data-collection software. Options include: Logger Pro 3.8.6.2, LabQuest App 2.1, LabQuest App 1.7.1, and Spectral Analysis 4 or newer. The latest version of software is available as a free download here: https://www.vernier.com/downloads/
4. Set the spectrometer to collect data in absorbance vs wavelength mode, if not already done.
5. Calibrate the spectrometer with the appropriate solvent.
6. Collect a spectrum with a known sample where the absorbance falls between 0.1 and 1.0 absorbance units.

RELATED TIL ENTRIES
Most Common:
TIL 3194: What cuvettes can I use in the Vernier UV-Vis or Fluorescence/UV-VIS Spectrophotometer?
TIL 3707: What is the Z dimension or beam height of your Spectrometers?
TIL 1850: Why are there blank data table cells in my spectrometer absorbance data?
TIL 2589: Why is the absorbance reading on my device (spectrometer/colorimeter) unstable or nonlinear at values above 1.0?

General:
TIL 2016: What is the difference between the types of cuvettes you sell for Spectrometers and the Colorimeter?
TIL 1846: Does the Vernier Spectrophotometer Optical Fiber work with any of the Ocean Optics Spectrometers or vice versa?
TIL 3194: What cuvettes can I use in the Vernier UV-Vis or Fluorescence/UV-VIS Spectrophotometer?
TIL 3596: What is the beam height for the Vernier UV-VIS Spectrophotometer?
TIL 3707: What is the Z dimension or beam height of your Spectrometers?
TIL 3957: The LED indicator light on my Vernier UV-VIS Spectrometer (VSP-UV) is red.

Software Issues:
TIL 2066: Why is the maximum sample time for a spectrometer set at 1000 milliseconds?
TIL 1850: Why are there blank data table cells in my spectrometer absorbance data?
TIL 2408: My spectrometer is connected to a computer and I get the error message, "Could not collect values from device," during calibration.
TIL 2853: Is there a way to toggle on/off the colored background behind the absorbance spectrum graph?
TIL 2354: My UV-VIS Spectrometer is not working properly. It won't calibrate or it is not reporting across the proper wavelength range?

Experiments:
TIL 1577: Do you have labs written for your spectrophotometers?
TIL 1563: Can I do blackbody experiments with the Vernier or Ocean Optics Spectrometers?
TIL 1683: Do you have a spectrometer that can measure DNA concentration and purity?
TIL 2404: Can I use my spectrometer to collect data at one wavelength (kinetics or Beer's law)?
TIL 2578: How do I get an A260/A280 value with your UV-vis spectrometers in Logger Pro?
TIL 2589: Why is the absorbance reading on my device (spectrometer/colorimeter) unstable or nonlinear at values above 1.0?

SPECIFICATIONS
Dimensions 18.5 cm x 17.0 cm x 7.0 cm
Power supply AC adapter (included)
Power consumption 3A start-up, 500 mA continuous
Light source deuterium (UV) and incandescent (VIS)
Detector Linear CCD
Wavelength range 220 to 850 nm
Wavelength reporting interval 1 nm
Wavelength accuracy* +/- 2.0 nm
Photometric accuracy** +/- 5.0%
Typical scan time 2 s
Operating temperature 15 to 35 degC
Optical resolution*** 4.0 nm (at 656 nm); 3.5 nm (at 486 nm)
*As determined with holmium oxide NIST standard
**As determined with potassium dichromate NIST standards from 0.1 to 1.0 AU
***As determined from the FWHM of the designated spectral peak of Hydrogen

CALIBRATION
You must calibrate the spectrometer every time you use Absorbance or %T mode. Calibration is not required for fluorescence or intensity.

RELATED VERNIER PRODUCTS
Vernier Fluorescence/UV-VIS Spectrophotometer, order code VSP-FUV, $2,899
Go Direct® SpectroVis® Plus Spectrophotometer, order code GDX-SVISPL, $399
Vernier Spectrometer, order code V-SPEC, $1,799
Replacement Power Supply for UV-VIS and Fluorescence Spectrometer, order code VSP-UV-PS, $35
Quartz Cuvettes, order code CUV-QUARTZ, $189
Plastic Cuvettes (UV-VIS), order code CUV-UV, $129

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