When testing a Tris-Compatible Flat pH Sensor, it is best to measure a buffer solution because it is easier to determine if the sensor is reading correctly. Do not test your sensor by measuring distilled water. Distilled water can have a pH reading in the range of 5.5–7.0, due to varying amounts of dissolved carbon dioxide. Furthermore, due to a lack of ions, the pH values reported with the sensor in distilled water will be erratic.
If your Tris-Compatible Flat pH Sensor is reading differently from the pH of a buffer solution (e.g., reads 6.7 in a buffer 7), you may simply need to calibrate the sensor. See the Calibration section for more information.
If your readings are off by several pH values, the pH readings do not change when moved from one buffer solution to a buffer solution of different pH, the sensor was stored dry for an extended period of time, or the sensor’s response seems slow, the problem may be more serious. A method called “shocking” can be used to revive pH electrodes. To shock your pH Sensor, perform the following:
- Soak the pH Electrode for 4–8 hours in an HCl solution of 0.1 M–0.5 M.
- Rinse off the electrode and soak the tip in freshly prepared long‑term storage solution (recipe above) for 30–60 minutes.
- Rinse the electrode and test it with buffer solutions of known pH.
Occasionally, mold will grow in the pH 4 buffer/storage solution. Mold will not harm the electrode and can easily be removed using a mild detergent solution. Mold growth in the storage solution can be inhibited by adding a buffer preservative.
For additional troubleshooting and FAQs, see www.vernier.com/til/1361