O2 Gas Sensor User Manual

Order Code: O2-BTA

The O2 Gas Sensor can be used to monitor gaseous oxygen levels in a variety of biology and chemistry experiments. The sensor is intended for measuring gaseous, not aqueous, O2 concentrations.

Note: Vernier products are designed for educational use. Our products are not designed nor are they recommended for any industrial, medical, or commercial process such as life support, patient diagnosis, control of a manufacturing process, or industrial testing of any kind.

What's Included

  • O2 Gas Sensor
  • 250 mL gas sampling bottle (Nalgene bottle with lid)

Compatible Software and Interfaces

Choose a platform below to see its compatibility requirements.

InterfaceLabQuest App
LabQuest® 3Full support
LabQuest 2 (discontinued)Full support
LabQuest (discontinued)Full support
InterfaceGraphical Analysis App for Computers
Version 5.4
Logger Pro
Version 3.16.2
Logger Lite
Version 1.9.4
LabQuest MiniFull supportFull supportFull support
LabQuest® 3Full supportFull supportFull support
LabQuest 2 (discontinued)Full supportFull supportFull support
LabQuest StreamFull support 1Full supportFull support 1
Go!LinkFull supportFull supportFull support
LabQuest (discontinued)Full supportFull supportFull support

Compatibility Notes

  1. Connect LabQuest Stream via USB. Wireless connection is not supported.
InterfaceGraphical Analysis App for Chrome
Version 5.4
LabQuest MiniFull support
LabQuest® 3Full support
LabQuest 2 (discontinued)Full support
LabQuest StreamFull support 1
Go!LinkFull support
LabQuest (discontinued)Full support

Compatibility Notes

  1. Connect LabQuest Stream via USB. Wireless connection is not supported.
InterfaceGraphical Analysis App for iOS
Version 5.4
Graphical Analysis GW for iOS
Version 4.0.6
LabQuest StreamFull supportFull support
LabQuest® 3Full support 1Full support 1
LabQuest 2 (discontinued)Full support 1Full support 1

Compatibility Notes

  1. iOS and Android devices can only connect to LabQuest 2 or LabQuest 3 via Wireless Data Sharing.
InterfaceGraphical Analysis App for Android
Version 5.4
Graphical Analysis GW for Android
Version 3.2
Google Science Journal
Version 1.2.259
LabQuest StreamFull supportPartial support 1Incompatible
LabQuest® 3Full support 2Full support 2Incompatible
LabQuest 2 (discontinued)Full support 2Full support 2Incompatible

Compatibility Notes

  1. Data can be collected using the sensor’s default calibration only.
  2. iOS and Android devices can only connect to LabQuest 2 or LabQuest 3 via Wireless Data Sharing.
Vernier Arduino® Interface ShieldFull support
Version 1.1
Version 2.0
NXT Sensor AdapterFull supportFull support
InterfaceNI LabVIEW
SensorDAQ (discontinued)Full support
Vernier myDAQ AdapterFull support 1
Go!LinkFull support
LabQuest MiniFull support
LabQuest StreamFull support
LabQuest® 3Full support
LabQuest 2 (discontinued)Full support
LabQuest (discontinued)Full support

Compatibility Notes

  1. You can only read the raw count/raw voltage output by this sensor. You must do the programming to convert to proper sensor units.
Texas Instruments
Version 2.4/4.0/5.2
Version 1.15
TI-84 SmartViewDataQuest
Version 4.2
TI-Nspire Software
EasyLinkFull support 1IncompatibleFull support 2Full supportFull support 2
CBL 2Full support 3Full support 3 4IncompatibleIncompatibleIncompatible

Compatibility Notes

  1. Use with TI-84 Plus calculators only.
  2. Requires an Easy to Go! adapter
  3. CBL 2 and LabPro interfaces cannot be used with TI-84 Plus CE calculators.
  4. DataMate cannot be used with color screen TI-84 Plus calculators; use EasyData with these calculators.

Getting Started

  1. Connect the sensor to the interface (LabQuest Mini, LabQuest 2, etc.).
  2. Start the appropriate data-collection software (Logger Pro, Logger Lite, LabQuest App) if not already running, and choose New from File menu.

The software will identify the sensor and load a default data-collection setup. You are now ready to collect data.

If you are collecting data using a Chromebook™, mobile device such as iPad® or Android™ tablet, or a Vernier wireless sensor or interface, please see the following link for up-to-date connection information:

Using the Product

Connect the sensor following the steps in the Getting Started section of this user manual. Note: For best results, keep the sensor upright as much as possible during use. Reading may drift if used in a horizontal position.



For many measurements, it will not be necessary to calibrate the O2 Gas Sensor. The sensor is shipped with a stored calibration.

For more accurate measurements, the sensor can be calibrated at 0 and 20.9% oxygen. Follow the 2-point calibration procedure. For the first point, push and hold the zero button with a pointed object such as a straightened paper clip. The button is located on the side of the sensor next to CAL. Enter a value of 0 for this reading. Release the button and take a second reading. Enter a value of 20.9% oxygen or a corrected value from the table in the Effect of Humidity section of this user manual. Once finished, the sensor should now read 20.9% (or the value entered from the table). For detailed instructions on calibration see the related links below.

You can also store your custom calibration on the sensor using the following steps:

  1. Go to the Storage tab and click the Set Sensor Calibration button (Logger Pro) or Save Calibration to Sensor (LabQuest). Once saved, this new calibration will be used automatically, regardless of the interface to which the sensor is connected.
  2. Click Done to complete the calibration process.

• For instructions on calibration using Logger Pro computer software, see

• For instructions on calibration using LabQuest App, see

• For instructions on calibration using Graphical Analysis with a Chromebook, see

• For instructions on calibration using Graphical Analysis with an iOS or Android device, see

Effect of Humidity

Because the oxygen concentration varies with the amount of water vapor in the atmosphere, you may want to adjust your atmospheric oxygen calibration value to improve accuracy when using the O2 Gas Sensor. The accepted value of 20.9% for atmospheric oxygen levels is calculated in dry air (0% humidity). If you know the relative humidity of the location at which you are calibrating, you can substitute one of the values below in place of 20.9%.

Relative humidity






Oxygen in % by volume








0–27% (0–270 ppt)


± 1% volume O2 (at 760 mmHg)



Warm up time

< 5 seconds

Response time

90% of final reading in ~12 seconds

Output signal range

0 to 4.8 VDC; 2.7 to 3.8 VDC @ 21% O2

Output impedance

1 KΩ

Input voltage

5 VDC +/–0.25 VDC

Gas sampling mode


Normal operating temperature range

20 to 30°C

Storage temperature range

–20 to +60°C

Pressure range

0.5 to 1.5 atm

Pressure effect

Directly proportional

Vout = Vout (standard) × (P/1013)

Default calibration values

  • slope: 6.5625 %/V
  • intercept: 0%


  • Sensor tube: 45 mm length, 28 mm OD
  • Total length: 120 mm

Care and Maintenance

Important: The O2 Gas Sensor must be stored upright when not in use. This is necessary to maintain the sensor. Failure to store upright will shorten the life of the sensor.

As your O2 Gas Sensor ages, the readings will decrease. This is normal, as the chemicals in the electrochemical cell are depleted. It does not mean the sensor is no longer functional; rather, it simply requires that you perform a calibration and store it as described previously. Once the readings in air are below 12% prior to calibration, or 1.8 volts during calibration, the sensor should be replaced. You may choose to have your sensor refurbished and a new element installed, or you may purchase a new sensor.

For tips on how to extend the life of the O2 Gas Sensor, see

How the Sensor Works

The Vernier O2 Gas Sensor measures the oxygen concentration in the range of 0 to 27% using an electrochemical cell. The cell contains a lead anode and a gold cathode immersed in an electrolyte. Oxygen molecules entering the cell are electrochemically reduced at the gold cathode. This electrochemical reaction generates a current that is proportional to the oxygen concentration between the electrodes. The sensor output is a conditioned voltage proportional to the reaction current.


  • Even though the sensor responds rather quickly to changes in O2 concentration, the gas has to diffuse into the electrochemical cell located at the top of the sensor shaft before any changes in concentration can be detected. Because the diffusion of a gas is a slow process, there can be some delay in readings.
  • To collect data in a controlled environment, we recommend that you use the 250 mL Nalgene collection bottle that is included with your sensor. Place the tip of the sensor into the opening of the bottle and push the sensor into the bottle. When the sensor will go no further, you have a seal. Very important: Do not place the sensor into any liquid. The sensor is intended only for measuring gaseous, not aqueous, O2 concentration.
  • To collect data in a controlled environment and also use our CO2 Gas Sensor, we recommend purchasing either the BioChamber 250 (250 mL capacity) or the BioChamber 2000 (2 L capacity). Each BioChamber has a second, grommetted opening so that you can insert two probes at once.

Repair Information

If you have watched the related product video(s), followed the troubleshooting steps, and are still having trouble with your O2 Gas Sensor, contact Vernier Technical Support at or call 888-837-6437. Support specialists will work with you to determine if the unit needs to be sent in for repair. At that time, a Return Merchandise Authorization (RMA) number will be issued and instructions will be communicated on how to return the unit for repair.

For information on common repairs for the O2 Gas Sensor, see


Item Order Code

BioChamber 250


BioChamber 2000


250 mL Nalgene Bottle w/Lid


The BioChambers have two openings with grommets so that you can insert both the CO2 Gas Sensor and the O2 Gas Sensor to take measurements of the atmosphere inside the BioChamber simultaneously.


Vernier warrants this product to be free from defects in materials and workmanship for a period of five years from the date of shipment to the customer. This warranty does not cover damage to the product caused by abuse or improper use. This warranty covers educational institutions only.

Contact Support

Fill out our online support form or call us toll-free at 1-888-837-6437.